We then functionally validated key genes in a high-fat diet-induced T2DM mouse model, assessing gene expression and the therapeutic effects of electroacupuncture (EA) using RT-qPCR.<h4>Results</h4>SMR and colocalization analyses identified <i>PLXNB2</i> and <i>LTBP3</i> as core candidate genes associated with T2DM. The gene discussed is LTBP3; the disease is type 2 diabetes mellitus.