EIF5A and melanoma: Furthermore, High EIF5A expression was significantly associated with poor patient outcomes (p < 0.001), Immunofluorescence analysis showing significantly elevated EIF5A expression in melanoma tissues compared to controls (p < 0.01).<h4>Conclusion</h4>This study reveals the functional heterogeneity of melanoma cells and their interactions with the immune microenvironment, identifies key subpopulations, prognostic signatures, and EIF5A as a plausible prognostic biomarker candidate and potential therapeutic target that warrants mechanistic validation in melanoma.