<h4>Background & aims</h4>We investigated whether liver disease alters HSD17B13 isoform expression, identifying selective loss of exon 2-skipped variants and uncovering variant B as a structured, noncoding RNA with silencing potential.<h4>Methods</h4>Human liver samples from lean control (n = 6), metabolic dysfunction-associated steatotic liver (MASL, n = 8), and metabolic dysfunction-associated steatohepatitis (MASH, n = 8) participants were analyzed by isoform-specific reverse-transcription PCR and quantitative PCR. The gene discussed is HSD17B13; the disease is metabolic dysfunction-associated steatohepatitis.