Additionally, the expression levels of CPT1A, FABP5, MAP2K3, MAP2K1, and HRAS were upregulated, whereas the expression of CYP27A1 was downregulated, which was consistent with the proteomic findings, indicating that these targets have been validated as key proteins associated with the molecular pathology of flexural atopic dermatitis lesions in antecubital and popliteal fossa regions. Here, CYP27A1 is linked to atopic eczema.