AKT1 and colorectal carcinoma: Western blot analysis showed that EEAL decreased the protein expression of AKT, P-mTOR, and HIF-1, while upregulating the expression of the autophagy marker LC3B.<h4>Conclusion</h4>This study demonstrates that EEAL exerts its anticancer effects against CRC by inhibiting the AKT/mTOR/HIF-1 pathway, leading to oxidative stress and mitochondrial dysfunction, which subsequently triggers excessive autophagy and compromises cell membrane integrity.