ACHE and Alzheimer disease: Acetylcholinesterase inhibitors (AChEIs) have been shown to mitigate AD symptoms, yet traditional AChEI screening approaches remain time-consuming and inefficient.<h4>Results</h4>To address this limitation, we developed multi-species AChEI screening network (MAISNet), an AChEI screening framework based on acetylcholinesterase (AChE) data from six species.