<i>In vivo</i>, WLBA3 reduced inflammation and oxidative stress by inhibiting the NLRP3 inflammasome pathway and modulating gut microbiota (e.g., <i>Lactobacillus</i> and <i>Alistipes</i>), which in turn significantly improved weight gain and fatigue resistance, attenuated cognitive function, and anxiety-like behavior. Here, NLRP3 is linked to Anxiety.