To investigate the functional role of PRKD3 in cell proliferation, its expression was knocked down using siRNA transfection, followed by proliferation assays.<h4>Results</h4>Immunohistochemical analysis revealed that PRKD3 protein was predominantly localized in the cytoplasm of both colorectal cancer and adjacent normal epithelial cells (sampled from > 5 cm beyond the tumor margin). The gene discussed is PRKD3; the disease is colorectal cancer.