Finally, hub gene expression was validated through reverse-transcription quantitative PCR (RT-qPCR).<h4>Results</h4>Seven hub genes (<i>TMEM150B</i>, <i>TNIP1</i>, <i>ATRN</i>, <i>FAAH</i>, <i>FBXW4</i>, <i>RAX</i>, and <i>WNT8B</i>) were identified as uniquely mutated in HL specimens, with <i>TMEM150B</i> exhibiting the highest mutation frequency. This evidence concerns the gene TMEM150B and Hodgkins lymphoma.