Genomic DNA extracted from peripheral blood samples was analyzed for thrombophilia-associated polymorphisms, including <i>F5</i> Leiden (G1691A), <i>F2</i> G20210A, <i>MTHFR</i> C677T, <i>MTHFR</i> A1298C, <i>SERPINE1</i> 4G/5G, and <i>F13A1 V34L</i>, using a real-time PCR-based Bosphore<sup>®</sup> Thrombophilia Panel. The gene discussed is MTHFR; the disease is Rare hereditary thrombophilia.