Western blotting analysis showed that EPA attenuates the NF-κB and AKT/mTOR signaling pathways, promotes the expression of cellular stress-related proteins, and inhibits the expression of EMT-related proteins in both cell lines.<h4>Conclusions</h4>EPA exhibits potent anti-tumor activity against USC cells and enhances the efficacy of carboplatin. Here, AKT1 is linked to neoplasm.