CD8A and neoplasm: In addition, <i>in vitro</i> cellular assays such as CCK-8, scratch assay and Transwell verified that RHOB could promote the proliferation, migration and invasion of ESCC cells <i>in vitro</i>.<h4>Conclusion</h4>This study constructed molecular subtypes related to CD8+ T cells and analyzed in depth the biological characteristics, genomic variation, and role of these three molecular subtypes in the tumor microenvironment and immunotherapy.