CD8A and esophageal squamous cell carcinoma: Our research aims to construct novel molecular subtypes through the gene expression profiling of CD8+ T cells during differentiation and to predict the prognostic and therapeutic effects in ESCC patients.<h4>Methods</h4>In the single-cell sequencing (scRNA-seq) analysis, we clustered and visualized cell subsets using the Seurat package, removed batch effects between samples using the Harmony package, and performed pseudo-time analysis of CD8+ T cells using the Monocle2 package.