However, the pathological mechanisms underlying the temporal sequence of H1N1-Mp co-infection remain poorly characterized, and relevant animal models are lacking.<h4>Methods</h4>In this study, we established a model of influenza H1N1 and <i>Mycoplasma pneumoniae</i> co-infection in Syrian hamsters and infected two pathogens in interval of 72 hours. Here, PPP1CB is linked to coinfection.