Functional validation was conducted in radiotherapy-resistant CRC cell lines through loss-of-function experiments including proliferation, migration/invasion, and radiosensitivity assays.<h4>Results</h4>FKBP10 emerged as the only gene achieving statistical significance in both discovery (log2FC = 0.74, <i>p</i> = 0.0007) and validation (log2FC = 0.52, <i>p</i> = 0.032) cohorts among 48 radioresistance-associated genes. This evidence concerns the gene FKBP10 and colorectal carcinoma.