Though observed in various cancers, their distribution and role in tumor immunity are not fully understood.<h4>Methods</h4>To investigate this, a multiplex cyclic immunofluorescence assay was developed to detect and spatially analyze Tph cells in tumors.<h4>Results</h4>This revealed three CXCL13-expressing CD4<sup>+</sup> T-cell subsets: Tfh, Tph, and a 'C-C motif chemokine receptor 2 (CCR2)<sup>-</sup> Tph-like' population which, in contrast to the classically CCR2-enriched Tph phenotype described in inflamed tissues, shows markedly reduced CCR2 expression. This evidence concerns the gene CXCL13 and cancer.