Spatial profiling showed CCR2<sup>-</sup> Tph-like cells embedded in an immunoregulatory network, marked by CD69 and inhibitory checkpoints B7-H3 and PD-L1 on surrounding cells.<h4>Conclusions</h4>This dual signaling suggests CCR2<sup>-</sup> Tph-like cells may modulate tumor immunity by balancing activation and suppression, with potential implications for checkpoint blockade therapy. Here, CD69 is linked to neoplasm.