Using transient expression in N2a neuroblastoma cells, we employed a comprehensive functional approach-including luciferase reporter assays, Western blotting, immunofluorescence, and RT-qPCR-to assess protein expression levels, subcellular localization, their interaction with known corepressors (TLE1 and CtBP1), and their transcriptional activity on selected <i>ARX</i>-known targets. The gene discussed is TLE1; the disease is neuroblastoma.