Moreover, the expression of prognostic genes was validated in different Non-small cell lung cancer (NSCLC) cell lines through reverse transcription quantitative polymerase chain reaction (RT-qPCR).<h4>Results</h4>Seven prognostic genes (<i>VDAC1</i>, <i>TXNRD1</i>, <i>GDF15</i>, <i>TRIB3</i>, <i>LPL</i>, <i>KCNQ1</i>, <i>PKP2</i>) were identified, RT-qPCR assays confirmed that these genes exhibited significant expression differences in different NSCLC cell lines. Here, TRIB3 is linked to non-small cell lung carcinoma.