The succinylated PEI-9, characterized by low toxicity and high gene transfer efficiency, was then fabricated and characterized on HCT-116 cancer cells and normal Vero cell lines.<h4>Results</h4>systems biology studies revealed guanylin's aberrant expression patterns, methylation variations, and mutational changes as well as its remarkable association with immune engagement and poor survival outcomes in CRC. The gene discussed is GUCA2A; the disease is colorectal carcinoma.