RNA was isolated from urine sediment for RT-qPCR to determine roundabout guidance receptor 1, corticotropin releasing hormone, and insulin-like growth factor 2 expression and calculate the uRNAp bladder cancer probability score.RESULTSIn the detection cohort, 547 samples were collected from 529 patients. This evidence concerns the gene ROBO1 and urinary bladder carcinoma.