CFTR and cystic fibrosis: The previously uncharacterised <i>CFTR</i> variants p.Ser364Pro and p.Phe1078Ser exhibit diminished expression in HEK293 cells, substantiating their pathogenic nature, with p.Phe1078Ser responding positively to the <i>in vitro</i> treatment with CFTR-modulator molecules.<h4>Conclusion</h4>This study demonstrates the potential of long-read sequencing using ONT as an efficient means to detect CF-causing variants in African populations.