<h4>Objective</h4>This study aims to investigate the regulatory mechanism by which the acidic microenvironment modulates LAMC2 expression in non-small cell lung cancer (NSCLC) and to elucidate its roles in tumor cell proliferation, migration, invasion, and associated signaling pathways, thereby providing a theoretical foundation for targeted lung cancer therapies.<h4>Methods</h4>A cohort of 104 pathologically confirmed NSCLC patients treated at a designated hospital from January 2018 to December 2021 was enrolled. This evidence concerns the gene LAMC2 and lung cancer.