While SDC4 has been previously implicated as a key driver for regulating myofibroblast activity in mechanically induced fibrosis in cardiac tissue, its specific role and shedding activity in chicken fibroblasts in relation to WB myopathy remain poorly understood.<h4>Methods</h4><i>In vitro</i> the overexpression system was used to mimic the previously detected increased SDC4 levels in WB and to further investigate fibrotic markers and syndecans at the gene and protein levels. Here, SDC1 is linked to myopathy.