(-)-Gallocatechin gallate and indomethacin bound well to GZMB, while benzo(a)pyrene and benzo(e)pyrene had good binding potential with LSP1.<h4>Conclusions</h4>We established lactylation as a critical regulator of AML, and GZMB and LSP1 were identified as lactylation-related clinical modeling indicators, which provides a foundation for choosing prognostic and therapeutic strategies for AML. Here, LSP1 is linked to acute myeloid leukemia.