qRT-PCR and immunohistochemistry were performed to detect GZMB/LSP1 expression in AML patients.<h4>Results</h4>Seven hub LRGs were identified in the AML groups: LSP1, MPO, GZMB, SPINK2, HLA-DRB1, HLA-DRA and POU2F2, of which GZMB and LSP1 passed MR test. Here, GZMB is linked to acute myeloid leukemia.