This study aims to investigate whether CaSR regulates SLC26A6 expression through a specific signaling pathway, thereby playing a role in experimental calcium oxalate kidney stone formation in rats.<h4>Methods</h4><i>In vivo</i>, a calcium oxalate kidney stone model was established in Wistar rats by intragastric administration of 1% ethylene glycol (E.G.,) and 1% ammonium chloride. The gene discussed is SLC26A6; the disease is Calcium oxalate nephrolithiasis.