SI and type 2 diabetes mellitus: Free binding energy calculations (MM-PBSA and MM-GBSA) further suggested that luteolin had stronger, more stable interactions with amylase and sucrase compared to glucosidase.<h4>Conclusion</h4>This study provides a comprehensive computational evaluation of luteolin derived from Caralluma tuberculata, offering detailed insights into its enzyme-specific interactions with key carbohydrate hydrolyzing enzymes relevant to type 2 diabetes.