SI and diabetes mellitus: <h4>Introduction</h4>Caralluma tuberculata, a medicinal plant from the Apocynaceae family, has been traditionally used to manage diabetes due to its rich secondary metabolite content.<h4>Methodology</h4>This study employed LC/ESI-MS/MS analysis to identify bioactive compounds in C. tuberculata, followed by in silico screening for their inhibitory effects on key carbohydrate-digesting enzymes-alpha-amylase, sucrase, and alpha-glucosidase associated with type 2 diabetes.