DNMT3A and acute myeloid leukemia: We developed a Local Promoter Methylation Disorder (LPMD) algorithm to quantify methylation instability and evaluate its clinical utility in predicting azacitidine response.<h4>Methods</h4>The LPMD algorithm was developed using the GSE62298 dataset (68 AML patients: 15 DNMT3A-mutant, 53 wild-type) to quantify local methylation heterogeneity through a 1-kb sliding window across CpG sites.