This study aims to bridge basic and clinical research by validating G1's efficacy in ameliorating MASLD-related hepatic steatosis, exploring its molecular mechanisms, and clarifying GPER1's association with human MASLD.<h4>Methods</h4>We investigated the expression of GPER1 in human liver tissue and its correlation with the severity of steatosis. Here, GPER1 is linked to Hepatic steatosis.