We sought to investigate the correlation between epileptic seizures of different duration and elevated Nf-L levels as a biomarker for neuroaxonal injury also considering methodological confounders in Nf-L measurement.<h4>Methods</h4>In adult patients, Nf-L was investigated in serum and/or cerebrospinal fluid (CSF) after a single epileptic seizure (51 patients), acute repetitive seizures (11 patients) or status epilepticus (20 patients) along with standard acute CSF parameters. This evidence concerns the gene NEFL and status epilepticus.