IRF3 and viral infectious disease: Together, these findings reveal a dual regulatory mechanism by which avian TRIM25 activates IRF7 to elicit robust type I interferon (IFN-I) responses, providing a molecular explanation for rapid antiviral immunity in species lacking IRF3.<h4>Importance</h4>Most avian species, including those in the orders <i>Galliformes</i> and <i>Anseriformes</i>, lack IRF3; consequently, IRF7 plays a pivotal and more comprehensive role in regulating innate immunity against viral infections.