UPLC-MS/MS analysis and molecular docking identified Hesperidin, Quercitrin, and Carnosol as major bioactive compounds in TAE with high binding affinity for PTEN.<h4>Conclusions</h4>In conclusion, our findings demonstrate that TAE exerts protective effects against epithelial senescence in DKD by modulating the PTEN/AKT/mTOR signaling, highlighting its potential as a novel therapeutic approach for the management of DKD. This evidence concerns the gene PTEN and diabetic kidney disease.