<h4>Objectives</h4>To determine and compare the expression of matrix metalloproteinases (MMPs) 2, 9, tissue inhibitors of metalloproteinase (TIMPs) 1, 2, cystathionine β-synthase (CBS), specificity protein 1 (Sp1), and microRNA-22 (miR-22) in the early-onset preeclampsia (EOPE) patients and normotensive controls at both transcriptional and translational levels.<h4>Methods</h4>The study was carried out on EOPE women (n=50) as cases, and normotensive, non-proteinuric pregnant women (n=50) as controls, enrolled at the Department of Obstetrics and Gynaecology. Here, SP1 is linked to preeclampsia.