STRING and KEGG analysis showed that PFN-1 and TGLN-2 both displayed association with regulation of actin cytoskeleton.<h4>Conclusion</h4>Aberrantly expression of actin binding proteins, PFN-1 and TAGLN-2 and tumor suppressor gene PTEN in pre and post-NAC patients in different tumor stages compared with healthy individuals can provide valuable prognostic information and may have potential as a therapeutic target. This evidence concerns the gene PFN1 and neoplasm.