The results of the qRT-PCR study indicated that CCND1, CXCL8, MYC, and VEGFA were upregulated in CRC cell lines, an observation consistent with existing public database records.<h4>Conclusions</h4>Six butyrate metabolism-related biomarkers (CCND1, CXCL8, MMP3, MYC, TIMP1, and VEGFA) were screened out to provide a basis for exploring the prediction of CRC diagnosis. The gene discussed is MMP3; the disease is colorectal carcinoma.