This study was to compare the effectiveness of 7 major let-7-5p isoforms (let-7a to let-7g) to regulate ABCC5 and LIN28B targets and inhibit HCC cell viability in vitro by using novel bioengineered RNA let-7-5p (BioRNA/let-7-5p) agents. Here, ABCC5 is linked to hepatocellular carcinoma.