PLAT and Stroke: In this study, we hypothesised that endothelial tPA plays a key role in modulating the microglial response and maintaining blood brain barrier (BBB) integrity after stroke.<h4>Methods</h4>Using a mouse model with endothelial-specific deletion of tPA (VeCad<sup>Cre</sup> - tPA<sup>Flox</sup>), combined with a thrombotic stroke model and high-resolution imaging, we investigated the effects of endothelial tPA on vascular inflammation and microglial activation.<h4>Results</h4>Our results demonstrate that microglia-vessel contacts increase post-stroke.