We report a novel cachexia-associated satellite cell subcluster, comprising 71.1% of the population at 3.5 weeks, corresponding with a +20.33% increase in cell size (p = 0.0266) and +19.73% increase in the proportion of activated PAX7<sup>+</sup>MYOD<sup>+</sup> cells after 24 h cultured on individual myofibres (p = 0.0226). Here, MYOD1 is linked to Cachexia.