Targeted massively parallel sequencing of 158 deafness-related genes was performed, and individuals with variants related to <i>MYO7A</i>-associated low-frequency hearing loss were identified.<h4>Results</h4>Among 18,475 hearing loss patients, we identified 60 patients from 44 unrelated families carrying five variants (p.[Asn140Lys; Glu1835Gln], p.Leu479Pro, p.Leu656Val, p.Gly660Arg, and p.Arg668His) for <i>MYO7A</i>-associated low-frequency hearing loss. This evidence concerns the gene MYO7A and hearing loss disorder.