Variants including R197G, Y170N, and T151K in the PDX1 Protein were considered the highest deleterious mutants.<h4>Conclusion</h4>These findings will provide insight into the molecular mechanisms by which PDX1 mutations may contribute to β-cell dysfunction and diabetes development and offer a rational framework for prior-itizing variants for experimental validation and clinical interpretation. The gene discussed is PDX1; the disease is diabetes mellitus.