FOS and Anxiety: Immunohistochemical staining for c-Fos expression and RNA sequencing of the medial prefrontal cortex (mPFC) were performed to evaluate neural activation and transcriptomic alterations.<h4>Results</h4>I-LT and ID-LT were associated with reduced anxiety-like behaviors in the OFT and EPM compared with untreated SPS-S-exposed rats (p < 0.001).