<b>Objectives:</b> This study evaluated the test-retest repeatability of semi-quantitative and volumetric features derived from artificial intelligence (AI)-assisted lesion segmentation on <sup>18</sup>F-DCFPyL Prostate Specific Membrane Antigen (PSMA)-PET/CT imaging of patients with prostate cancer (PCa). The gene discussed is FOLH1; the disease is prostate cancer.