Overall, fNIRS studies reported increased activation in the left dorsolateral prefrontal cortex after art therapy, and studies on HR, skin conductance, salivary cortisol, sAA, IL-6, CRP, and RSA illustrated its positive effects in reducing stress, anxiety, and sad mood.<h4>Discussion</h4>However, existing research has primarily addressed emotional pain, with no studies assessing its impact on physical pain in healthy populations using objective physiological or biological measures, showing that there is a gap for assessing physical pain improvement by art therapy. This evidence concerns the gene CRP and Anxiety.