IFNG and tuberculosis: These results indicate that MPT64 is a specific M. tuberculosis antigen with significant potential for TB diagnosis through multiple approaches, including IFN-γ release assays, skin tests, and serological detection, particularly when combined with CLE for enhanced diagnostic performance.<h4>Importance</h4>MPT64 is a protein from the tuberculosis (TB) bacterium that triggered the strongest immune response among seven new antigens tested.