Overall, gene-based comparison of rare coding variants indicated an enrichment in several genes, including <i>TP53</i>, <i>CREBBP</i>, and <i>DNMT3A</i>.<h4>Conclusions</h4>Rare P/LP germline variants were more frequent among glioma patients than in the reference population within our predefined gene set. Here, TP53 is linked to glioma.