Real-time PCR (RT-qPCR) confirmed low levels of <i>CASP7</i>, <i>PRKCB</i>, and <i>ENDOG</i> mRNA in CRC tissues, with no significant difference between <i>LMNB2</i> and <i>GZMB</i>.<h4>Conclusion</h4>These findings highlight 5 MPTDN-associated prognostic genes in CRC, providing insights for individualized treatment and prognosis. The gene discussed is CASP7; the disease is colorectal carcinoma.