NR0B1 and Adrenal insufficiency: <h4>Objective</h4>To characterize the molecular spectrum of NR0B1 variants associated with X-linked congenital adrenal hypoplasia (X-hypoAC) in a multicenter Brazilian cohort and to highlight the clinical implications of early molecular diagnosis.<h4>Methods</h4>The authors investigated 12 unrelated families referred to pediatric endocrinology centers across Brazil with a clinical diagnosis of early-onset adrenal insufficiency.