Conversely, several key genes were significantly downregulated, including <i>PIK3R1</i>, <i>FYN</i>, <i>CEBPA</i>, <i>MLXIPL</i>, <i>PPARA</i>, <i>CD36</i>, <i>PCK2</i>, <i>PNPLA3</i>, <i>NR1H4</i> and <i>MGLL</i>, which were involved in lipid metabolism, immune regulation and non-alcoholic fatty liver disease (NAFLD) pathways. The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.