Traditional markers like WBC and lymphocyte percentage showed high discrimination against non-specific fever (AUC = 0.973-0.989).<h4>Conclusion</h4>This study shows that CD64 is an efficient biomarker for differentiating scrub typhus from febrile diseases, offering crucial evidence for establishing rapid diagnostic pathways and holding value for early triage of febrile patients. This evidence concerns the gene FCGR1A and scrub typhus.