Functionally, restoring WT1 expression in PCa cells potently suppressed malignant behaviors, inhibiting proliferation and migration while triggering apoptosis.<h4>Conclusion</h4>This study defines a novel SPI-WT1 axis, positioning WT1 as a druggable, immune-correlated biomarker in prostate cancer. Here, WT1 is linked to posterior cortical atrophy.