WT1 and posterior cortical atrophy: Finally, molecular docking and <i>in vitro</i> gain-of-function assays (proliferation, migration, clonogenicity, and apoptosis) in DU145 and PC-3 cell lines were conducted to validate functional mechanisms.<h4>Results</h4>Network pharmacology identified WT1 as a central regulatory node in the SPI-PCa interaction network.