Various parameters were then measured and compared between the two groups, including alexithymia, depression, anxiety, sleep quality, personal and social performances, self-care ability, quality of life, serum levels of orphanin FQ and interleukin-2, incidence of adverse reactions, and nursing satisfaction.<h4>Results</h4>At both 4 and 8 weeks post-intervention, all measured parameters improved significantly from the baseline in both groups (<i>p</i> < 0.05). This evidence concerns the gene PNOC and depressive symptom measurement.