<h4>Background</h4>Junctophilin 3 (JPH3) acts as a tumor suppressor in several cancers; however, the role of JPH3 in anaplastic thyroid cancer (ATC) is still unknown.<h4>Methods</h4>Via bioinformatics prediction and verified through Western blot experiments, the expression level of JPH3 in ATC was ascertained. This evidence concerns the gene JPH3 and thyroid gland undifferentiated (anaplastic) carcinoma.